Trypan blue exclusion assay by flow cytometry

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Trypan blue exclusion assay by flow cytometry

Dye exclusion tests are used to determine the number of live and dead cells. These assays are based on the principle that intact plasma membranes in live cells exclude specific dyes, whereas dead cells do not. Although widely used, the trypan blue (TB) exclusion assay has limitations. The dye can be incorporated by live cells after a short exposure time, and personal reliability, related to the...

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Anemia with positive direct Coombs' test induced by trypan blue.

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ژورنال

عنوان ژورنال: Brazilian Journal of Medical and Biological Research

سال: 2014

ISSN: 1414-431X,0100-879X

DOI: 10.1590/1414-431x20143437